2 non-VGI 34.1 19.6 −14.5 non-VGII 32.1 18.8 −13.3 non-VGIII 16.9 28.8 11.9 VGIV VGIV Table 5 VGII subtyping SYBR MAMA Ct Rapamycin molecular weight values and genotype assignments for VGIIa,b,c   VGIIa_Assay_45211 VGIIb_Assay_502129 VGIIc_Assay_257655 Isolate ID Strain type via MLST VGIIa Ct Mean non-VGIIa Ct Mean Delta Ct Type call via assay VGIIb Ct Mean non-VGIIb Ct Mean Delta Ct Type call via assay VGIIc Ct Mean non-VGIIc Ct Mean Delta Ct Type call via assay Final Call B6864 VGIIa 17.2 30.5 13.3 VGIIa 31.0 17.5 −13.5 non-VGIIb 40.0 27.8 −12.2 Ulixertinib ic50 non-VGIIc VGIIa B7395 VGIIa 19.8 33.5 13.7 VGIIa 33.1 20.3 −12.9 non-VGIIb 40.0 30.6 −9.4 non-VGIIc VGIIa B7422 VGIIa 18.3 33.6 15.4 VGIIa 26.4 17.6 −8.8 non-VGIIb

39.2 28.6 −10.6 non-VGIIc VGIIa B7436 VGIIa 18.6 31.7 13.1 VGIIa 30.1 17.0 −13.2 non-VGIIb 38.0 29.1 −8.9 non-VGIIc VGIIa B7467 VGIIa 20.5 37.3 16.8 VGIIa 35.1 20.3 −14.7 non-VGIIb 40.0 30.9 −9.1 non-VGIIc VGIIa B8555 VGIIa 17.1 31.2 14.1 VGIIa 30.3 17.5 −12.8 non-VGIIb 40.0

27.7 −12.3 non-VGIIc VGIIa B8577 VGIIa 20.8 36.8 16.0 VGIIa 32.8 20.8 −12.1 non-VGIIb 40.0 31.4 −8.6 non-VGIIc VGIIa B8793 VGIIa 15.1 29.8 14.7 VGIIa 30.7 18.6 −12.1 non-VGIIb 40.0 29.8 −10.2 non-VGIIc VGIIa B8849 VGIIa 19.8 34.4 14.6 VGIIa 33.6 20.2 −13.4 non-VGIIb 40.0 30.6 −9.4 non-VGIIc VGIIa CA-1014 VGIIa 13.1 27.3 14.2 VGIIa 27.0 14.0 −13.0 non-VGIIb 34.9 24.2 −10.7 non-VGIIc VGIIa CBS-7750 VGIIa 21.8 32.2 10.4 VGIIa 33.4 21.5 −11.9 non-VGIIb 40.0 34.1 −5.9 non-VGIIc VGIIa ICB-107 VGIIa 21.8 33.6 11.8 VGIIa 33.2 21.2 −12.0 non-VGIIb 40.0 33.8 −6.2 non-VGIIc VGIIa NIH-444 VGIIa 14.8 27.3 12.5 VGIIa 28.5 15.3 −13.1 non-VGIIb Palbociclib purchase 36.1 25.7 −10.3 non-VGIIc Anidulafungin (LY303366) VGIIa B8508 VGIIa 17.0 27.8 10.8 VGIIa 26.5 17.3 −9.2 non-VGIIb 31.7 22.7 −9.1 non-VGIIc VGIIa B8512 VGIIa 17.6 28.1 10.4 VGIIa 26.3 18.0 −8.3 non-VGIIb 33.2 24.2 −9.0 non-VGIIc

VGIIa B8558 VGIIa 16.3 24.8 8.5 VGIIa 27.3 15.3 −12.0 non-VGIIb 29.4 20.0 −9.4 non-VGIIc VGIIa B8561 VGIIa 15.8 27.5 11.8 VGIIa 25.0 16.9 −8.1 non-VGIIb 33.4 23.2 −10.2 non-VGIIc VGIIa B8563 VGIIa 14.5 27.3 12.8 VGIIa 23.9 15.6 −8.3 non-VGIIb 31.7 21.7 −10.0 non-VGIIc VGIIa B8567 VGIIa 15.0 36.2 21.2 VGIIa 24.5 16.0 −8.5 non-VGIIb 31.8 22.2 −9.5 non-VGIIc VGIIa B8854 VGIIa 14.7 26.7 12.0 VGIIa 24.1 15.1 −9.0 non-VGIIb 31.4 22.2 −9.2 non-VGIIc VGIIa B8889 VGIIa 17.0 28.1 11.0 VGIIa 25.9 17.3 −8.7 non-VGIIb 33.2 23.8 −9.4 non-VGIIc VGIIa B9077 VGIIa 16.7 27.8 11.1 VGIIa 25.6 16.7 −9.0 non-VGIIb 32.9 24.4 −8.4 non-VGIIc VGIIa B9296 VGIIa 17.0 27.5 10.5 VGIIa 25.5 17.3 −8.2 non-VGIIb 32.9 24.8 −8.1 non-VGIIc VGIIa B7394 VGIIb 40.0 19.0 −21.0 non-VGIIa 17.3 29.6 12.3 VGIIb 40.0 29.0 −11.0 non-VGIIc VGIIb B7735 VGIIb 31.0 18.3 −12.8 non-VGIIa 18.7 31.3 12.6 VGIIb 38.1 28.9 −9.3 non-VGIIc VGIIb B8554 VGIIb 32.9 21.2 −11.7 non-VGIIa 22.2 35.0 12.8 VGIIb 40.0 30.4 −9.6 non-VGIIc VGIIb B8828 VGIIb 31.9 21.1 −10.8 non-VGIIa 19.9 35.1 15.2 VGIIb 40.0 30.5 −9.5 non-VGIIc VGIIb B8211 VGIIb 27.8 16.9 −10.9 non-VGIIa 17.4 28.8 11.4 VGIIb 32.3 22.3 −10.0 non-VGIIc VGIIb B8966 VGIIb 26.