1B, mean = 5200). Variability in the level

of infection obtained between individual animals may have affected the capacity of the vaccine trial described here to achieve statistical significance between some of the different treatment groups. In the study undertaken by Flisser et al. [4] pigs were given eggs isolated from gravid T. solium segments such that individual animals received directly comparable challenge infections. In the trial of TSOL45-1A where statistically significant protection was achieved [4] the twelve control animals harboured between 6 and 127 cysts, representing a range varying by a factor of 21 from lowest to highest. In Peru where the trial described here was undertaken, greatest success has been achieved in experimental Selleckchem JAK inhibitor infections in pigs by giving whole gravid proglottids rather than isolated eggs, however a disadvantage of the method is the necessity to use different adult worms Small molecule library cost to supply the proglottids and individual animals also receiving different proglottids

[28]. In the experiment described here, this led to a variation in the levels of infection in controls by a factor of 174 between the lowest and highest values (22–3831 cysts). In this case, it is difficult to interpret whether the TSOL45-1A vaccinated animals that had 25 and 63 cysts were either non-protected or >98% protected depending on whether they received the lower or higher infective dose delivered to the control animals. Nevertheless TSOL16 appeared to be a more effective immunogen than TSOL45-1A in this experiment, with TSOL16-vaccinated animals being both statistically significantly protected in comparison to controls as well as having statistically significant fewer cysts than the TSOL45-1A vaccinates (P < 0.05). The oncosphere antigens of cestode parasites are typically problematic ADP ribosylation factor to express in E. coli [19], [29] and [30] and GST or MBP fusion proteins have been used as immunogens because these have advantages in regard to expression level and solubility compared to the non-fused or HIS-tagged antigens. Here we used

a vaccination strategy incorporating both GST and MBP fusion proteins of the same antigen in an attempt to boost immune responses to the parasite-derived portion of the recombinant antigens. The first two immunizations given to the pigs each contained the oncosphere antigens fused to GST. The third immunizations each contained the antigens fused to MBP, the aim being to boost immune responses to the parasite-encoded portions of TSOL16, TSOL45-1A or TSOL45-1B rather than to the GST fusion partner. Previous studies have shown that a substantial portion of the antibody response in pigs [17] and sheep [31] and [32] is raised against the highly immunogenic GST fusion partner. Responses to both TSOL16 and TSOL45-1A were substantially greater after the third immunization compared with responses after the second ( Fig. 1).