Although lyn–/–IL-21–/– mice lacked anti-DNA IgG, they still developed GN. The remaining IgG antibodies specific for non-DNA self-Ags have pathogenic potential since they recognize dissociated glomerular basement membrane and RNA-containing Ags. Indeed, IgG deposits were present in four of four lyn–/–IL-21–/– kidneys examined. Inflammation initiated by these non-DNA IgG autoantibodies could then be amplified by positive feedback loops between cytokine-producing T cells and CD11b+Gr1+CD11c− myeloid cells in the periphery [49, 50] and by elevated CD11b+

and CD8+ cells in the kidney, none of which are significantly altered by IL-21-deficiency. We find that the majority of splenic IL-21 mRNA is produced by CD4+ T cells in an IL-6-dependent manner in both WT and lyn–/– mice, consistent with previous reports [15-17, CH5424802 in vivo 39], IL-6 is required for expansion of Tfh cells and/or their expression of IL-21 upon chronic, but not acute, lymphocytic choriomeningitis

virus infection [56, 57]. These observations suggest that IL-6 maintains steady-state levels of IL-21 expression by T cells basally and during chronic infection or autoimmunity, while IL-6-independent events can induce IL-21 selleck chemicals during acute responses to certain pathogens or Ags. Kidney damage in lyn–/– mice is abrogated by deficiency of IL-6, but not IL-21 [11, 12]. Thus, IL-6 has both IL-21-dependent and -independent functions in the autoimmune phenotype of lyn–/– animals. There are several mechanisms by which IL-6 could drive MycoClean Mycoplasma Removal Kit the latter events. IL-6 promotes Th17-cell development and inhibits Treg-cell activity [58]. We observed a slight increase in Th17 cells among CD4+ T cells in lyn–/– mice (WT 0.34 ± 0.04%, n = 5 versus lyn–/– 1.25 ± 1.09%, n = 4), although this was not significant. Treg cells are present in lyn–/– mice but fail to suppress disease [53]. IL-6-deficiency also promotes myelopoiesis [59] and likely contributes to the increase in myeloid cells and their role in proinflammatory feedback loops in lyn–/– mice [12, 49, 50]. Finally, IL-6 acts on endothelial cells to alter

homing of leukocytes to sites of inflammation [60]. This may contribute to kidney damage in lyn–/– mice. Disruption of IL-21 signaling also prevents IgG autoantibody production and reduces ICOS+CXCR5− T cells in BXSB.Yaa [31] and MRL.lpr mice [33, 34]. However, a more profound effect on other aspects of the autoimmune phenotype was observed in BXSB.Yaa and MRL.lpr mice lacking the IL-21R than was seen in lyn–/–IL-21–/– mice [31, 34] In contrast, IgG autoantibody production is independent of IL-21 in Roquinsan/san mice [46], despite increased Tfh cells and IL-21 overexpression. This varying dependence of autoimmune phenotypes on IL-21 signaling may be explained by different disease mechanisms in each model.