Adacolumn selective granulocytapheresis (GCAP) has been associated with clinical efficacy 3-MA cell line in patients with UC. In the present study

we sought the effect of sequential GCAP procedures in peripheral blood APCs in patients with UC and the effect on soluble cytokines. Methods:  We used multiparametric flow cytometry to quantify peripheral blood APCs and serum cytokines in 210 samples obtained from seven patients with steroid-dependent or steroid resistant UC undergoing GCAP treatment. Samples were drawn before, after 30 and 60 min of each session. Results:  Each GCAP session resulted in a dramatic tenfold reduction of peripheral blood CD16-mDC (P < 0.01), pDC decreased twofold (P = 0.05) but CD11c-mDC remained unchanged. This depletion was reached after 30 min and maintained

at 60 min. The depletion of CD16-mDC and monocytes was associated with a reduction of serum tumor necrosis factor levels and a raise in interleukin-10 levels, although no statistical difference was reached. Conclusion:  The effect of GCAP in peripheral blood APC consisted mainly on a significant depletion of tumor necrosis factor-α secreting CD16-mDC. This finding could suggest a potential mechanism of GCAP beneficial effect that must be confirmed in larger series. “
“End-stage liver disease and hepatocellular carcinoma from chronic hepatitis C (HCV) remain as the most common indications MG-132 cost for liver transplantation (LT) in the Western world. Unfortunately, HCV infection universally 上海皓元 persists into the post-transplant period, threatening graft and patient survival. Unlike chronic HCV in the immunocompetent population, the natural history of chronic HCV in the LT population has a more accelerated course, with 10%-30% of LT recipients progressing to cirrhosis within 5 years of

transplantation and more than 40% within 10 years. The median interval of developing cirrhosis is 9.5 years from transplantation, as compared to 30 years from infection in immunocompetent persons.[1] Undoubtedly, recipients with recurrent HCV have a lower graft and patient survival than their noninfected counterparts.[2] Various factors associated with aggressive HCV recurrence after LT have been identified (Fig. 1). Donor age >40 years,[3] higher HCV RNA levels at time of transplantation and in the early posttransplant period,[1, 3] and use of corticosteroid pulses or antilymphocyte antibody preparations, such as OKT3, for treatment of acute cellular rejection have predicted fibrosis progression and, consequently, graft and patient survival.[1, 3, 4] Underlying all these factors is the recipient’s immune response, which exerts its actions through both innate and adaptive mechanisms.