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Infect Dis 2006,43(5):556–563.CrossRefPubMed 42. Bouzari S, Jafari A, Azizi A, Oloomi M, Nataro JP: Short report: characterization of enteroaggregative Escherichia coli isolates from Iranian children. Am J Trop Med Hyg 2001,65(1):13–14.PubMed 43. Okeke I, Lamikanra A, Czeczulin J, Dubovsky F, Kaper J, Nataro J: Heterogeneous virulence of enteroaggregative Amine dehydrogenase Escherichia coli strains isolated from children in Southwest Nigeria. J Infect Dis 2000, 181:252–260.CrossRefPubMed 44. Cerna JF, Nataro JP, Estrada-Garcia T: Multiplex PCR for detection of three plasmid-borne genes of enteroaggregative Escherichia coli strains. J Clin Microbiol 2003,41(5):2138–2140.CrossRefPubMed 45. Carver TJ, Rutherford KM, Berriman M, Rajandream MA, Barrell BG, Parkhill J: ACT: the Artemis Comparison Tool. Bioinformatics 2005,21(16):3422–3423.CrossRefPubMed Authors’ find more contributions AS co-conceived the study, designed and coordinated the work, contributed to reading HEp-2 adherence assay slides, and provided significant input into writing the manuscript. LRM-S performed and read HEp-2 adherence assays, performed DNA hybridizations and maintained and mined strain databases. JNF contributed to reading HEp-2 adherence assay slides and made contributions to writing the manuscript. INO co-conceived the study, performed sequence analyses, designed and validated the PCR-RFLP and wrote the first draft of the manuscript.

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