As an important contribution to the understanding of the botanical distribution over the geographical region, in CAD3, the Clidemia pollen type was identified only as minor pollen, and in SAD2 and CAD1, it was identified as a secondary pollen ( Table 1). The distribution of the Clidemia pollen may be associated with features that limit the bee’s access to flowers of this genus, or its incidence may not be prevalent in these counties. In spite of presenting the Serjania (Sapindaceae) as the predominant pollen type, SAD2 displayed Clidemia as a secondary pollen, however it corresponding to 33.8%. In

the honey collected in SAD3, the Myrcia, Myrtaceae family, pollen type was identified with a 77.6% occurrence rate, and SAD3 PD-0332991 chemical structure PD0332991 was classified as monofloral. Pollen from the genus Myrcia is often found in palynological analyses of Melipona bee products in the Amazon ( Marques-Souza, 1996). The Clidemia pollen type was not found, but the collection location was away from urban areas and located in the Ituxi-Lábrea Indian extractive reserve, which displays characteristic native vegetation. CAD1 and CAD3 were classified as multifloral because they contained two and four secondary pollen types, respectively ( Table 1). The analyses of correlation based on the pollen type results showed a dendrogram

of similarity with a distribution of four clusters (A, B, C and D). The cluster A including the closely related samples SAD1, CAD2 and CAD4, where CAD4 and SAD1 showed more similarity among themselves than with sample CAD2. The similarity observed in this group is a consequence of Chlormezanone the predominance of the Clidemia pollen type ( Table 1). The samples SAD2 and CAD1 formed cluster B, which showed weak similarity with cluster A and high degree of differences with the samples CAD3

and SAD3, distributed in clusters C and D, respectively. In the cluster B the Clidemia pollen type also was found, however in minor quantities when compared to the cluster A. The negative correlation of CAD3 with all other samples probably is because only in this sample was identified the pollen from Tapirira guianensis (Anacardiaceae); this same behaviour in SAD3 could be explained by the identification of Myrcia pollen as predominant only this sample. In the Brazilian Amazon, Meliponini species, including M. s. merrillae, have exploited floral sources such as Byrsonima, Euterpe, Maximiliana, Mimosa, Myrcia, Schefflera and Solanum ( Marques-Souza, 1996). In a palynological work focused on pollen stored by 23 Meliponini species along the Rio Negro channel, Amazonas ( Rech & Absy, 2011), the species cited as having been exploited by M. s. merrillae (and the other Meliponini) included related Alchornea, Byrsonima (subsp. Cephalotrigona), Cecropia (subsp.