a, b Pustules. c–i Conidiophores (Hairs visible in e). DNA-PK inhibitor j Conidia. All from SNA. All from G.J.S. 00–72. Scale bars: a = 1 mm, b = 0.25 mm; c–e = 20 μm; f–i = 10 μm Fig. 10 Trichoderma gillesii, Hypocrea teleomorph. a, b Stroma morphology. c Stroma surface, macro view.
d Stroma surface, micro view. e–g Perithecia, median longitudinal sections showing surface region and internal tissue of stroma. h, i Asci. j Part-ascospores. Note the subglobose part-ascospores in Figs. i and j All from G.J.S. 00–72. Scale bars: a, b = 1 mm; c = 0.5 mm; d, g = 20 μm; e = 50 μm, f = 100 μm; h–j = 10 μm MycoBank MB 563905 Trichodermati sinensi Bissett, Kubicek et Szakacs simile sed ob conidia anguste ellipsoidea, 3.2–4.0 × 1.7–2.2 μm differt. Holotypus: BPI 882294. Teleomorph: Hypocrea sp. Optimum temperature for growth on PDA and SNA 25–35°C; after 72 h in darkness with intermittent light colony on PDA completely or nearly completely filling a 9-cm-diam Petri plate (slightly slower at 35°C); within 96 h in darkness with intermittent light colony radius on SNA 40–50 mm (slightly faster at 35°C). Conidia forming on PDA within 48–72 h at 25–35°C in darkness with intermittent light; after 1 week on SNA at 25°C under light. No diffusing pigment noted on PDA. Colonies grown on SNA for 1 week at 25°C under light slowly producing pustules. Pustules formed of intertwined hyphae, individual conidiophores not evident, slowly turning green. Conidiophores arising from hyphae of the pustule,
typically comprising a strongly developed main axis with fertile lateral branches and often terminating in a sterile terminal extension (‘hair’). Hairs conspicuous, short, stiff erect, Protein Tyrosine Kinase inhibitor sterile, blunt, septate. Fertile branches increasing in length from the tip of the conidiophore, often paired, rebranching to produce either solitary phialides or unicellular
secondary branches; secondary branches terminating in a whorl of 3–5 divergent phialides. Intercalary phialides not seen. Phialides lageniform, nearly obovoidal, typically widest below the middle, (4.0–)4.5–7.0(−9.5) μm long, (2.2–)2.5–3.0(−3.2) μm at the widest point, base (1.2–)1.5–2.0(−3.0) μm wide, L/W (1.4–)1.5–2.5(−3.5) μm, arising from a cell (1.7–)2.0–3.0(−3.7) μm wide. Conidia ellipsoidal, (3.0–)3.2–4.0(−4.5) × (1.5–)1.7–2.2(−2.5) μm, L/W (1.4–)1.5–2.2(−2.5), green, smooth. Chlamydospores not observed. Teleomorph: Stromata brown, discoidal, CYTH4 margins slightly free, 3–4 mm diam, cespitose and covering an area ca. 15 mm diam, surface plane to undulate, GANT61 ic50 conforming to the surface of the substratum and adjacent stromata, ostiolar openings appearing as minute black papillae, no reaction to 3% KOH, ostiolar area greenish in lactic acid. Cells of the stroma surface in face view pseudoparenchymatous, ca. 5.5 × 4.5 μm diam, slightly thick-walled. Perithecia elliptical in section, 220–250 μm high, 130–190 μm wide, ostiolar region formed of small cells and gradually merging with the cells of the surrounding stroma surface.