Effect of solvents on (i) the cell morphology and structure (ii) surface hydrophobicity Fedratinib mouse and (iii) permeability of cell membrane have been examined using transmission electron microscopy, atomic force microscopy and other biochemical

techniques. The results show that (i) less hydrophobic (low log P-value) solvent cyclohexane alters the cell membrane integrity and (ii) cells adapt to organic solvents by changing morphology, size, permeability and surface hydrophobicity. However, no such changes were observed in the cells grown in tetradecane.

Conclusions:

It may be concluded that P. aeruginosa PseA responds differently to solvents of different hydrophobicities. Bacterial cell membrane is more permeable to less

hydrophobic solvents that eventually accumulate in the cytoplasm, while highly hydrophobic solvents have lesser tendency to access the membrane.

Significance and Impact of the Study:

To the best of our knowledge, these are first time observations that show that way of bacterial solvent adaptability depends on nature of solvent. Difference in cellular responses towards solvents of varying log P-values (hydrophobicity) might prove useful to search for a suitable solvent for carrying out whole-cell biocatalysis.”
“Aim:

To study the effect of biosurfactant on aqueous phase solubility and biodegradation of chlorpyrifos.

Methods and Results:

A Pseudomonas sp. (ChlD), isolated from agricultural soil by enrichment culture technique in the presence of chlorpyrifos, was capable JQ-EZ-05 research buy of producing biosurfactant (rhamnolipids) and degrading chlorpyrifos (0 center dot 01 g l(-1)). The partially purified rhamnolipid biosurfactant preparation, having a CMC of 0 center dot 2 g l(-1), was evaluated for its ability to enhance aqueous phase partitioning and degradation of chlorpyrifos (0 center dot 01 g l(-1)) by ChlD strain. The best degradation efficiency was observed at 0 center dot 1 g l(-1) supplement of biosurfactant, as validated by GC and HPLC studies.

Conclusion:

The addition

of biosurfactant at 0 center dot 1 g l(-1) resulted in more than 98% this website degradation of chlorpyrifos when compared to 84% in the absence of biosurfactant after 120-h incubation.

Significance and Impact of the Study:

This first report, to the best of our knowledge, on enhanced degradation of chlorpyrifos in the presence of biosurfactant(s), would help in developing bioremediation protocols to counter accumulation of organophosphates to toxic/carcinogenic levels in environment.”
“Aims:

To determine whether the extracellular products (ECPs) from Aeromonas hydrophila, a frog bacterial pathogen that is resistant to skin antimicrobial peptides of three different frog species Xenopus laevis, Litoria aurea and Litoria raniformis, can modulate the activity of these peptides.