Furthermore, pharmacokinetics of 4Gal-liposomes studied in rat and tissue distribution was carried out by in vivo imaging. Finally, the analysis of frozen sections of liver was carried out to be able to review the mechanism of the targeting capacity of 4Gal-liposomes to liver tissue. The outcomes propose that the compound described in this function could serve being a useful tool for learning hepatic endocytosis, and it is a suitable carrier for site-specific drug delivery to your liver. DTPA was bought from Aladdin Chemistry Co Ltd . DSPE and DSPC were purchased from Genzyme Corporation . Anhydrous pyridine was purchased from Sigma Chemical Co . 2,3,4,6-Tetra-O-acetyl–D-galactopyranosyl bromide was obtained from J&K Scientific Co Ltd . HepG2 cells and Hela cells have been bought from the Laboratory Animal Center of Sun Yat-sen University .
Cells had been cultured in Dulbeccos Modified Eagles medium supplemented with 10% fetal bovine serum and antibiotics at 37C in humidified air with 2% carbon dioxide. All other chemicals have been of reagent grade. Male Kunming selleckchem purchase Sunitinib mice and male Sprague Dawley rats had been obtained from the Laboratory Animal Center of Sun Yat-sen University. All experimental procedures had been approved and supervised by the Institutional Animal Care and Use Committee of Sun Yat-sen University. Synthesis of 4Gal-DTPA-DSPE conjugates 4Gal-DTPA-DSPE was synthesized by the following procedure : activation of DTPA, connection of DTPA and DSPE, galactosylation of DTPA-DSPE, and removal of protection from hydroxyl groups. In the synthetic process, the carboxyl groups of DTPA have been firstly activated by the acetic anhydride dissolved in anhydrous pyridine.23 Then the amino group of DSPE was covalently linked to a carboxyl group of DTPA.
17 The next step was to connect the remaining carboxyl groups of DTPA and 1-hydroxyl group of Gals .24 Finally, the protecting groups of why not try these out hydroxyl groups had been removed selectively.25 The detailed synthetic routes from the compound are depicted in Supplementary material. The structure of 4Gal-DTPA-DSPE and intermediate products was characterized by 1H-NMR and mass spectrometry . Preparation and characterization of liposomes DSPC, Chol, and 4Gal-DTPA-DSPE were dissolved in CHCl3 and dried under an N2 stream. A trace amount of CHCl3 was removed by keeping the lipid film under a vacuum. The lipid film was hydrated with 250 mM 2SO4 to obtain a blank liposome suspension. The liposome suspension was then sequentially extruded through polycarbonate membranes with a pore size of 200 nm and 100 nm.
The resulting liposomes were dialyzed against phosphate-buffered saline at 37C. For drug loading, DOX was dissolved in a small volume of deionized water and added towards the liposomes to achieve a drug:lipid ratio of 1:10 . The loading process was carried out at 65C for 30 minutes, and DOX liposomes were obtained.