In this study it was evaluated the effect of L-NAME and naltrexone in the antinociception induced by administration of gabapentin in the orofacial formalin test of mice. Methods: The algesiometer assay was performed by the administration of 20 pi of 2% formalin solution injected into the upper right lip of each mouse. Results: The dose of gabapentin that produces
the 50% of the maximum possible effect (ED50) was significantly increased by the pretreatment with L-NAME or naltrexone. Conclusions: These results suggest that gabapentin produce antinociception partly via the activation nitridergic pathways and opioid 3-MA PI3K/Akt/mTOR inhibitor system. (C) 2014 Institute of Pharmacology, Polish Academy of Sciences. Published by Elsevier Urban &Partner Sp. z o.o. All rights reserved.”
“Lactobacillus pentosus strain S-PT84 isolated from Kyoto pickles enhances splenic natural killer (NK) cell activity and exhibit anti-allergic effects by modulating the Th1/Th2 (T-helper1/T-helper2) balance. In the present study,
we investigated whether the immune response could LY2606368 be activated by intranasal administration of S-PT84 in the respiratory immune system and protected against influenza virus infection in mice. When BALB/c mice received intranasal administration of S-PT84 once daily for 3 consecutive days, S-PT84 strongly induced interleukin-12 (IL-12) and gamma interferon (IFN-gamma) production in mediastinal lymph node (MLN) cells. At intranasal infection with influenza virus PR8 (a mouse-adapted H1N1 strain) after S-PT84 treatment, the survival rates of mice improved in a dose-dependent manner, and the titer of influenza virus in bronchoalveolar lavage fluids (BALF) was significantly decreased by S-PT84 administration. Production of IL-12 and this website alpha-interferon (IFN-alpha) in BALF
were significantly higher in mice treated with S-PT84 compared to the control mice. Lung NK activity was also significantly augmented in S-PT84-treated mice. These results suggested that the L pentosus strain S-PT84 showed inhibitory activity against influenza virus infection. (C) 2010 Elsevier B.V. All rights reserved.”
“A water-soluble crude polysaccharide (WPS) was obtained from Laminaria japonica by hot water extraction. Three major polysaccharide fractions (WPS-1. WPS-2 and WPS-3) were purified from WPS by anion-exchange chromatography. Monosaccharide components analysis indicated that galactose was the predominant monosaccharide in WPS and WPS-3, accounting for 56.25% and 54.11%, respectively. And fucose was the predominant monosaccharide in WPS-1 and WPS-2, accounting for 46.91% and 45.1%, respectively. Antioxidant activity tests revealed that WPS-2 showed significant function of scavenging hydroxyl free radical and WPS-1 exhibited the highest inhibitory effects on superoxide radical. Cytotoxicity of all polysaccharide fractions was evaluated by MTT assay and Hoechst 33258 staining.