The assay

was highly sensitive and 100% specific in both functions rendering it effective for H7 diagnosis. Exploiting H7 specific neutralizing Mab 62 and 98 makes the H7 dual ELISA a more specific Selleck GSK3 inhibitor and sensitive assay as compared to conventional immunological tests. A rapid test based on this H7 dual ELISA will serve as effective tools for both H7 diagnosis and surveillance investigation, meeting the needs to counter the ongoing outbreak of H7N9 in China. In conclusion, the dual-function ELISA presented in this study was proven to provide a fast, simple and cost-effective platform for both antigen and antibody detection. Exploiting H7 specific neutralizing Mab 62 and 98 makes the H7 dual ELISA a more specific and sensitive assay as compared to conventional immunological tests. A rapid test based on this H7 dual ELISA will serve as effective tools for both H7 diagnosis and surveillance investigation, meeting the needs to counter the ongoing outbreak of H7N9 in China. Acknowledgements This work was supported by Temasek Life Sciences laboratory, Singapore. We thank Mr. Subramanian Kabilan and Mr. Govindarajan for animal work and Dr. Tanja K. Kiener for proofreading. We are grateful for the reverse genetic viruses and plasmids contributed by Dr. Ruben Donis and ABT-199 manufacturer Dr. Li Mei Chen from the Centers for Disease Control

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