The correlation coefficient, slopes, Y-intercepts, and the regression equation of the calibration curve were determined and shown in Table 2. The % RSD was found to be <2.0% while the % recovery was found to be in the range of 97�C103%. Figure 3 Linearity Table 1 Linearity and range results Table 2 Results of different test parameters selleck screening library Accuracy Accuracy was studied using two different sets of three different solutions, containing 90, 100, and 120 ��g/mL of m-cresol. Each solution was spiked in the mobile phase and injected onto HPLC (n = 9). The % recovery was found to be between 98% and 102% and the % RSD was found to be <1.0% as shown in Table 3. Table 3 Accuracy results Precision Precision was evaluated based on intra-day (repeatability) and inter-day (intermediate precision) variation and on different columns.
The repeatability was assessed with six independent samples of 100 ��g/mL of m-cresol. Single injection from each preparation was injected and the results are shown in Table 4. The % RSD of the main peak area was found to be <2.0 %. Table 4 Repeatability results Using the experimental design and matrix as shown in Table 5, intermediate precision was evaluated on different days with different equipment and with different columns. Three replicate injections of system suitability standards (100 ��g/mL of m-cresol) prepared independently were considered for the study. Intra-day precision was determined for 100 ��g/mL of m-cresol by performing five different conditions as mentioned in Table 5 (n = 15) and RSD were calculated.
Table 5 Experimental design for intermediate precision The % RSD for the main peak area of m-cresol standard within each set and between different sets was found to be <2.0%. The % recovery of m-cresol standard was found to be between 95.0% and 105.0% within each set, and the maximum variation between sets was found to be 3.0%. The results are shown in Tables Tables1,1, ,3,3, and and44. Robustness Robustness is a measure of its capacity to remain unaffected by small, but deliberate variations in method parameters and provides an indication of its robustness during normal usage. Robustness was tested using two variables: age effect of the mobile phase and test samples. Freshly prepared samples (100 ��g/mL of m-cresol) and those stored for 7 days were analyzed using both the freshly prepared mobile phase and mobile phase stored for 7 days.
No variation in retention time was observed with percent variation from the initial day to 7 days. However, percentage variation of the principal peak area of m-cresol was found to be higher than 5% when compared to the principal peak area of m-cresol in specificity Dacomitinib samples. Due to variation in the peak area, it was decided to use freshly prepared sample as well as mobile phase before analysis.