The peptide markedly greater alkaline phosphatase activity in E1 and MSC cell cultures and decreased tartrate resistant acid phosphatase activity in RAW264 cell culture in a dose dependent manner, respectively. Additionally, the peptide stimulated mineralization evaluated by alizarin Caspase inhibition red staining in E1 and MSC cell cultures. The anabolic effect of WP9QY peptide was enhanced markedly by addition of BMP2. Increases in mRNA expression of IGF1, collagen style I, and osteocalcin had been observed in E1 cells treated with all the peptide for twelve and 96 h in GeneChip analysis. Addition of p38 MAP kinase inhibitor lowered ALP activity in E1 cells taken care of together with the peptide, suggesting a signal by means of p38 was involved in the mechanisms.

Conclusions: Taken collectively, the peptide abrogated osteoclastogenesis by blocking RANKL RANK signaling and stimulated Ob differentiation/ mineralization with unknown mechanism in vitro. Nonetheless, in our experimental circumstances the peptide exhibited bone anabolic result dominantly in vivo. Since the peptide is acknowledged to bind RANKL, we hypothesize the peptide exhibits the bone VEGFR signaling pathway anabolic action with reverse signaling as a result of RANKL on Obs. P21 T regs/Th17 function defect in systemic autoimmunity as a outcome of current thymic emigrants maturation defect Mark Goloviznin1, Natalia Lakhonina1, Alexander Yarilin2, Yulia Buldakova1, Vitaly Timofeev3, Tatiana Kremenchugskaya1, Marina Struchkova1 1Department of Inner Diseases of Dental Faculty, Moscow State University of Medication and Dentistry, Russia, 2Laboratory of Cell Immunology, Analysis Center Institute of Immunology, Moscow, Russia, 3Department of Faculty Treatment of Russian State Medical University, Moscow, Russia Arthritis Investigation & Treatment 2012, 14 21 T regs and Th17 cells are the new generation of CD4T cells which play crucial role in autoimmunity.

Both of subsets can influence each other and probably have common precursor. A key question for understanding Endosymbiotic theory the mechanism of autoimmunity is to recognize how T regs and Th17 cells turn from self protection to autoreactivity. Based on literature data and own observations, we have constructed a conception of age dependent thymic T cells maturation peripherialisation as cause of errors in Th17 T reg cells interrelations. The connection of T regs with thymus is determined currently. Connection of Th17 cells with thymus remains to be determined properly.

Main, there may be naturally occurring Tregs of thymic origin that are resistant to cell death and serve as reserve pool for autoimmunity protective suppressors. This mechanism could be affected by external factors pyruvate dehydrogenase activity producing profound lymphopenia. Previously we found that RA patients with numerous rheumatoid nodules and lymphopenia had statistically reliable decrease of CD3T cells level. We found definite negative correlation between CD3PBL amount and RN number. In all RA patients with and without RN we didnt found the decrease of CD4 receptor.