rubrum GlnD is regulated by alpha-ketoglutarate and divalent cations but not by glutamine. J Bacteriol 2007,189(9):3471–3478.PubMedCrossRef 12. Zhang Y, Pohlmann EL, Serate J, Conrad MC, Roberts GP: Mutagenesis and functional characterization of the four domains of
GlnD, a bifunctional nitrogen sensor protein. J Bacteriol 2010,192(11):2711–2721.PubMedCrossRef 13. Teixeira PF, Jonsson A, Frank BIBW2992 price M, Wang H, Nordlund S: www.selleckchem.com/products/cftrinh-172.html Interaction of the signal transduction protein GlnJ with the cellular targets AmtB1, GlnE and GlnD in Rhodospirillum rubrum: dependence on manganese, 2-oxoglutarate and the ADP/ATP ratio. Microbiology 2008, 154:2336–2347.PubMedCrossRef 14. Wang H, Franke CC, Nordlund S, Noren A: Reversible membrane association of dinitrogenase reductase activating glycohydrolase in the regulation of nitrogenase activity in Rhodospirillum Idasanutlin datasheet rubrum; dependence on GlnJ and AmtB1. FEMS Microbiol Lett 2005,253(2):273–279.PubMedCrossRef 15. Zhang Y, Wolfe DM, Pohlmann EL, Conrad MC, Roberts GP: Effect of AmtB homologues on the post-translational regulation of nitrogenase activity in response to ammonium and energy signals in Rhodospirillum rubrum. Microbiology 2006,152(Pt 7):2075–2089.PubMedCrossRef 16. Jiang P, Peliska JA, Ninfa AJ: Enzymological characterization of the signal-transducing uridylyltransferase/uridylyl-removing enzyme (EC 18.104.22.168) of Escherichia coli and its interaction with the PII
protein. Biochemistry 1998,37(37):12782–12794.PubMedCrossRef 17. Berthold CL, Wang H, Nordlund S, Hogbom M: Mechanism of ADP-ribosylation removal revealed by the structure and ligand complexes of the dimanganese mono-ADP-ribosylhydrolase DraG. Proc Natl Cepharanthine Acad Sci U S A 2009,106(34):14247–14252.PubMedCrossRef 18. Ormerod JG, Ormerod KS, Gest H: Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism. Arch Biochem Biophys 1961, 94:449–463.PubMedCrossRef 19. Bueno R, Pahel G, Magasanik B: Role of glnB and glnD gene products in regulation of the glnALG operon of Escherichia coli. J Bacteriol 1985,164(2):816–822.PubMed 20. Johansson M, Nordlund S: Purification of P(II)
and P(II)-UMP and in vitro studies of regulation of glutamine synthetase in Rhodospirillum rubrum. J Bacteriol 1999,181(20):6524–6529.PubMed 21. Atkinson MR, Kamberov ES, Weiss RL, Ninfa AJ: Reversible uridylylation of the Escherichia coli PII signal transduction protein regulates its ability to stimulate the dephosphorylation of the transcription factor nitrogen regulator I (NRI or NtrC). J Biol Chem 1994,269(45):28288–28293.PubMed 22. Hammarström A, Soliman A, Nordlund S: Low- and high-activity forms of glutamine synthetase from Rhodospirillum rubrum: sensitivity to feed-back effectors and activation of the low-activity form. Biochim Biophys Acta 1991,1080(3):259–263.PubMedCrossRef Competing interests The authors declare that they have no competing interests.