The signed scale free R2 plot analysis suggests that this selection has a good scale free topology fit, as the R2 value of 0. 85 indicates that the topology of the HLB response network is quite similar to most biological networks. The resulting citrus gene coexpression network contains 3,507 nodes with 56,287 edges. We next determined the robustness of our network across each dataset using the cross validation approach. We randomly left out one dataset and reconstructed the gene co expression networks using the remaining three datasets. The resulting four networks were then compared to the network based on all four datasets in terms of net work connectivity rank of each gene according Inhibitors,Modulators,Libraries to the sug gestion described elsewhere.

There were strong, highly significant connectivity correlations between the network based on all four data sets and the ones reconstructed from any combination of the three datasets. This suggests a high degree of preserva tion of gene co expression patterns Inhibitors,Modulators,Libraries across the networks based on different datasets. We then analyzed in detail the characteristics of the GSK-3 HLB response network. First, the frequency distribution of edges for each node was determined. As shown in Figure 2, the network contains 860 Probesets that are orphan nodes, 400 Probesets that have only one interaction, and the ma jority of the nodes that have at listed in Additional file 7. The p values of the overrepre sented GO terms were listed in Additional file 5. We also performed a GO enrichment analysis for the hub genes.

We arbitrarily divided Inhibitors,Modulators,Libraries the 2,247 hubs into two categories, minor hubs and major hubs and their overre presented GO terms were summarized in Additional file 8. The major hubs have 13 overrepresented GO terms, carbohydrate metabolic process, primary meta bolic process, metabolic process, secondary metabolic process, lipid metabolic process, cellular amino acid and derivative metabolic process, cellular process, localization, transport, establishment of localization, Inhibitors,Modulators,Libraries regulation of ana least three interactions and, by following Geisler Lee et al. are called hubs in this paper. Among the 2,247 Probesets, the majority have 3 100 edges, and the remaining 345 Probesets have 101 300 interactions, while only 1% have more than 300 interac tions. Overall, the mean number of interactions for each Probeset is 16, with the maximum of interactions being 369.

Cit. 4987. 1. S1 s at represents a gene most closely related to Arabidopsis SYP71 encoding a plant syntaxin which functions as a plasma membrane associated protein transporter. Second, we performed a GO enrichment analysis for the Probesets in the HLB response network. Among 30,173 Probesets, 22,775 have the Arabidopsis gene ID as their closest orthologs or homologs. Therefore, these Probesets were assigned GO terms based on the most recent Arabidopsis GO assignment.